This PhD project aims to investigate how DNA nanostructures with novel functional geometries can replicate key biochemical and mechanical features that support adaptability in living systems. Building on recent advances in DNA origami and computational design softwares, the project will explore novel nanoscale architectures capable of controlled shape transformation and mechanical actuation.

The goal is to build robot-like DNA assemblies with emphasis on shape morphing and mechanically responsive behavior. These robot-like DNA nanostructures will be designed to geometrically detect perturbations by incorporating, within the molecular system, proteins, or RNA to propose platforms to probe mechanobiological processes with molecular precision. In collaboration with the Ouldridge group, the project will combine oxDNA simulations for structural reshaping via strand-displacement reactions to facilitate the programming of dynamic structural features within 3D DNA origami. Structural and dynamic properties of the constructs will be characterized using cryo-electron microscopy and fluorescence-based approaches, including single-molecule FRET providing real-time insights into conformational changes.

This project offers the opportunity to contribute to the rational design of responsive biomimetic systems to help advancing our understanding of mechanical signaling at the nanoscale and for designing future biohybrid devices.